浙江省SARS冠状病毒分离与系统进化树分析

从浙江省3例SARS患者中收集含漱液标本，经处理后接种Vero、RD、VeroE6和Hep-2细胞进行病毒分离，培养3d后在Vero和RD细胞中可观察到细胞病变。从细胞培养上清中提取病毒核酸，用SARS冠状病毒特异性引物进行RT-PCR，并经测序证实从3份临床样本中分离到2株SARS冠状病毒株。对其中1株病毒的基因组进行了全序列测定并作系统进化树分析显示浙汀省SARS冠状病毒株与新加坡2774株和台湾TW1株最为接近。

Phylogenic Analysis of SARS-Coronavirus Isolated from Zhejiang Province

Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days. SARS-coronavirus RVA was extracted from supernatant of cell culture and RT-PCR was performed using SARS-coronavirus specific primers. Two strains of SARS-coronavirus were isolated from three clinical samples, which were identified by gene sequence. The complete genome sequence of one strain of SARS-coronavirus was determined and analysed by phylogenic tree. SARS-coronavirus isolated from the patients with SARS in Zhejiang province was almost homological to Singapore Sin2774 and Taiwan TW1.