Effect of endothelin-1(1-31) on intracellular free calcium in cultured human mesangial cells |
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Authors: | Yasuoka H Yoshizumi M Inui D Okishima N Houchi H Kirima K Oshita S Kido H Tamaki T |
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Institution: | Department of Pharmacology, The University of Tokushima School of Medicine, Japan. |
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Abstract: | We found that human chymase selectively produces 31-amino-acid length endothelins (1-31) (ETs(1-31)). We investigated the effect of synthetic ET-1(1-31) on intracellular free Ca2+ concentration (Ca2+]i) in cultured human mesangial cells. ET-1(1-31) increased Ca2+]i in a concentration-dependent manner to a similar extent as ET-1. The ET-1 (1-31)-induced Ca2+]i increase was not influenced by removal of extracellular Ca2+ but was inhibited by thapsigargin. ET-1(1-31)-induced Ca2+]i increase was not affected by phosphoramidon. It was inhibited by BQ123, but not by BQ788. These results suggest that ET-1(1-31) by itself exhibits bioactive properties probably through endothelin ET(A) or ET(A)-like receptors. Since human chymase has been reported to exist in the kidney, ET-1(1-31) may be a candidate substance for mesangium-relevant diseases. |
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