The production of D-acetoin by a transgenic Escherichia coli |
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| Authors: | Ui,Mimura,Okuma,& Kudo |
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| Affiliation: | Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu, Yamanashi,;Laboratory of Microbiology, The Institute of Physical and Chemical Research (RIKEN), Hirosawa, Wako, Saitama, Japan |
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| Abstract: | A 6 kbp SphI fragment encoding genes for the enzymes α-acetolactate synthase (ALS), α-acetolactate decarboxylase (ALDC) and meso -2,3-butanediol dehydrogenase ( meso -BDH), involved in the formation of meso -2,3-butanediol ( meso -BD) from pyruvic acid, was cloned into plasmid pUC118. When derivatives of this plasmid were introduced into Escherichia coli JM109, the transformants were able to produce D -acetoin ( D -AC) without contamination with L -acetoin ( L -AC). |
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