Expression of beta-tubulin during dormancy induction and release in apical and axillary buds of five woody species |
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| Authors: | Jan H W Bergervoet Hai-Chun Jing John W E van den Hout Renato Delmondez de Castro Bernard P A M Kunneman Raoul J Bino & Steven P C Groot |
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| Institution: | Centre for Plant Breeding and Reproduction Research, Department of Reproduction Technology, PO Box 16, 6700 AA Wageningen, The Netherlands; Tianjin Academy of Agricultural Science, Soil and Fertiliser Institute, Tianjin 300192, PR China; RIVM, PO Box 1, 3720 BA. Bilthoven, The Netherlands; Universidade Federal de Lavras, Departamento de Fitotecnia, Laboratorio de Sememtes, Cx. Postal 37, Lavras, MG, CEP 37200-000, Brasil; The Research Station for Nursery Stock, Boskoop, The Netherlands |
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| Abstract: | Cell cycle activity was studied in apical and axillary buds of Norway maple ( Acer platanoides L.), apple ( Malus ' M9 ') , pedunculate oak ( Quercus robur L.), Scots pine ( Pinus sylvestris L.) and rose ( Rosa corymbifera 'Laxa') during dormancy induction and release. Flow cytometric analyses revealed that in dormant buds, cells mainly were quiescent at the G0/G1 phase, while in non-dormant buds, a significantly higher frequency of G2 cells was found in all species. In western blots accumulation of 55 kDa beta -tubulin was found in active growing plant material, whereas in dormant buds the accumulation was much lower or below detection level. It was observed for all species that during dormancy induction the amount of beta -tubulin decreased, while during dormancy release a fast accumulation of beta -tubulin occurred. The dynamics of the beta -tubulin accumulation reflected the dormancy status of tree buds of the five species studied suggesting that the beta -tubulin level might be useful as a marker for the dormancy status in buds of temperate woody species. |
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