Biological activity of monomeric Sendai virus HN glycoprotein |
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| Authors: | Shunji Sugii |
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| Institution: | Department of Molecular Genetics, Antibioticos, S.A., Bravo Murillo 38, 28015-Madrid, Spain |
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| Abstract: | Abstract We have developed a transformation system for Streptomyces wadayamensis , a cephamycin C producer. 1?5 × 109 protoplasts can be obtained when late logarithmic phase cultures of this microorganisms are incubated with 10 mg/ml of lysozyme. Polyethylene glycol-Ca2+-mediated transformation of these protoplasts yielded 106 transformants per μg of pIJ702 or pIJ365 DNA. |
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| Keywords: | S wadayamensis protoplasts polyethylene glycol |
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