Chalcone synthases from spinach (Spinacia oleracea L.) |
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Authors: | L Beerhues H Robenek R Wiermann |
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Institution: | (1) Botanisches Institut der Westfälischen Wilhelms-Universität, Schlossgarten 3, D-4400 Münster, Federal Republic of Germany |
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Abstract: | The two chalcone-synthase forms from leaves ofSpinacia oleracea L. were purified to apparent homogeneity. Antibodies were raised against both proteins in rabbits. The specificity of the antibodies was tested using immunotitration, immunoblotting, and immunoelectrophoresis techniques. The antibodies exhibited exclusive specificity for chalcone synthase and did not discriminate between the two antigens. The homodimeric chalcone synthases had the same subunit molecular weight but differed in their apparent native molecular weights. The peptide maps indicated extensive homology between the proteins. Chalcone-synthase activity was not detected in isolated spinach chloroplasts. Both enzyme forms were present in spinach cell-suspension cultures in which they were induced by light.Abbreviations DEAE
diethylaminoethyl
- DTE
1,4-dithioerythritol
- EDTA
ethylenediaminetetraacetic acid
- HPLC
high-performance liquid chromatography
- IgG
immunoglobulin G
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Parts of the results were presented at the 14th International Botanical Congress at Berlin in July 1987 |
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Keywords: | Spinacia (chalcone synthase) Chalcone synthase (immunology peptides) Enzyme (different forms) |
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