Spin-trapping of free radicals formed during microsomal metabolism of ethylhydrazine and acetylhydrazine |
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| Authors: | O Augusto P R Ortiz de Montellano A Quintanilha |
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| Institution: | 1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, California 94143 USA.;2. Liver Center, University of California, San Francisco, California 94143 USA.;3. Membrane Bioenergetics Group, Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720 USA.;4. Department of Physiology/Anatomy, University of California, Berkeley, California 94720 USA. |
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| Abstract: | Radicals formed during oxidative metabolism of ethylhydrazine have been spin-trapped with PBN1. The trapped species has been identified as an ethyl radical by comparison of the ESR parameters of the PBN-adduct with (1) those of the analogous adduct formed during CuCl2-catalyzed oxidation of ethylhydrazine and (2) those reported for the adduct in the literature. The requirement for oxygen and NADPH, and the observation of a typical binding spectrum by difference spectroscopy, suggest the involvement of the cytochrome P-450 enzyme system in radical formation. Similar studies with acetylhydrazine demonstrate the generality of metabolic radical formation from hydrazines, although the radical trapped in this latter instance has not been identified. |
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