籼爪交水稻F_2群体的蒸煮食味品质研究

分析了籼型高直链淀粉恢复系CG133R与糯性爪哇稻22号杂交衍生的F2群体的蒸煮品质变异及其与淀粉粘滞性特征间的相关性,以及F2群体颗粒结合淀粉合成酶(Wxa基因)和可溶性淀粉合成酶(SSⅡ-3基因)主效基因的遗传。结果表明:蒸煮品质指标和RVA谱特征值在F2群体中广泛分离,其中变异最大的是消减值,其次为胶稠度、直链淀粉含量。高直链淀粉材料各理化指标与RVA谱特征值的相关性不显著;RVA谱特征值在中、低直链淀粉含量和糯稻群体中与各理化指标存在显著或极显著相关;在中、低直链淀粉材料中,RVA谱特征值与糊化温度(GT)也存在显著或极显著相关。用Wxa基因和SSⅡ-3基因的分子标记检测到这两个基因在F2群体中存在偏分离,分别指向两个亲本类型。除高直链淀粉材料外,可以通过RVA谱特征值来辅助筛选优质水稻品种。

Studies on Cooking and Eating Quality of F2 Population of Indica/Javanica Rice

A rice F2 population was obtained from a hybrid cross of the indica restorer line CG133R with high amylose content and the glutinous rice variety javanica 22, which was as materials. The variation and their correlation between the cooking and eating quality indices and starch RVA characteristic values were analyzed and the genetics of the major genes (Wx and SSII-3) controlling the physicochemical indexes in the F2 population were examined. The results indicated that the physicochemical indexes and RVA profile characteristics had great variation in F2 population, setback with the maximum variation, followed by gel consistency and amylose content; correlation of physicochemical indexes of materials with high amylose content were not significant with RVA profile characteristics. RVA profile characteristics in materials with medium, low amylose content and glutinous rice groups had significant or extremely significant correlation with the physical and chemical indices. In materials with medium and low amylose content, there also had significant or extremely significant correlation with gelatinization temperature (GT). Separation of Wx and SSII-3 genes in F2 population was detected using molecular marker and showed that the two molecular markers existed partial separation and deviated to two parent types, respectively. There was more variation in the F2 population except for the materials with high amylose content. RVA profile characteristics could be used for assisted-selection for quality rice breeding.