Cloning and characterisation of the cytochrome c gene of Aspergillus nidulans |
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| Authors: | Desmond C Raitt Rosemary E Bradshaw and Tim M Pillar |
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| Institution: | (1) Leicester Biocentre, Leicester University, University Road, LE1 7RH Leicester, UK;(2) Present address: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Porter Biosciences Building, Campus Box 347, 80309-0347 Boulder, CO, USA;(3) Present address: Department Microbiology and Genetics, Massey University, Private bag, Palmerston North, New Zealand;(4) Present address: Institut für Physiologische Chemie, Universitäts-Krankenhaus Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany |
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| Abstract: | The cytochrome c gene (cycA) of the filamentous fungus Aspergillus nidulans has been isolated and sequenced. The gene is present in a single copy per haploid genome and encodes a polypeptide of 112 amino acid residues. The nucleotide sequence of the A. nidulans cycA gene shows 87% identity to the DNA sequence of the Neurospora crassa cytochrome c gene, and approximately 72% identity to the sequence of the Saccharomyces cerevisiae iso-1-cytochrome c gene (CYC1). The S. cerevisiae CYC1 gene was used as a heterologous probe to isolate the homologous gene in A. nidulans. The A. nidulans cytochrome c sequence contains two small introns. One of these is highly conserved in terms of position, but the other has not been reported in any of the cytochrome c genes so far sequenced. Expression of the cycA gene is not affected by glucose repression, but has been shown to be induced approximatly tenfold in the presence of oxygen and three- to fourfold under heatshock conditions. |
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