Efficacy of bacterial auxin on in vitro growth of Brassica oleracea L. |
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Authors: | Basharat Ali Shahida Hasnain |
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Institution: | (1) Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan |
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Abstract: | Murashige and Skoog’s (MS) medium was supplemented with supernatant of Halomonas desiderata RE1 in different combinations to observe the impact of bacterial auxin on in vitro growth of Brassica oleracea L. Three groups of combinations MS + BS (Bacterial supernatant), MS + BS + 10% CW (coconut water) and MS + BS + 4 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) were considered. Different amounts of BS used in each combination were 50, 100, 150
and 200 μl in 5 ml MS medium. Media combinations inoculated with seeds, internodal explants and callus of B. oleracea L. were incubated in a growth chamber at 25 ± 1°C and exposed to 16-h cool fluorescent light. Seeds inoculated on MS + BS and
MS + BS + 10% CW, shoot elongation was observed over control whereas this response was suppressed in 2,4-D-containing media.
In explants inoculated on MS + BS, MS + BS + 10% CW and MS + BS + 4 mg l−1 2,4-D different responses such as callus induction, adventitious shoot induction and hypertrophy were observed at different
supernatant treatments. In callus inoculation, callus proliferation was observed in most of the treatments at different media
combinations. |
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Keywords: | Adventitious shoot induction Bacterial auxin Halomonas desiderata Hypertrophy Internodal explants |
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