| 5’核苷酸酶碱性磷酸酶双重染色法在毛细淋巴管研究中的应用 |
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| 引用本文： | 廖新波,唐慰萍,赵颖海,李飞虹.5’核苷酸酶碱性磷酸酶双重染色法在毛细淋巴管研究中的应用[J].中国组织化学与细胞化学杂志,1996(2). |
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| 作者姓名： | 廖新波 唐慰萍 赵颖海 李飞虹 |
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| 作者单位： | 广东医学院病理学教研室 湛江524023 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 本文应用5’-N-ALP双重染色法观察了裸鼠皮肤及人胃癌组织内淋巴管的形态和细微分布.在光镜下毛细淋巴管、淋巴管呈5’-N强阳性反应,管壁显示明显的棕色或深棕色,而毛细血管、血管的ALP呈强阳性,管壁呈明显的蓝色.据此可用组化方法将毛细淋巴管和毛细血管区别开来.本法能显示呈褐色的毛细淋巴管,特别是呈实性条索状的毛细淋巴管,因而双重染色比HE染色更能客观、准确地显示毛细淋巴管的分布.
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| 关 键 词： | 裸鼠皮肤 人胃癌组织 毛细淋巴管 5′核苷酸酶碱性磷酸酶双重染色 |
A STUDY OF 5'-NUCLEOTIDASE-ALKALINE PHOSPHATASE DOUBLE STAINING ON LYMPH CAPILLARIES |
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| Liao Xinbo,Tang Weiping,Zhao Yinghai,Li Feihaong.A STUDY OF 5'-NUCLEOTIDASE-ALKALINE PHOSPHATASE DOUBLE STAINING ON LYMPH CAPILLARIES[J].Chinese Journal of Histochemistry and Cytochemistry,1996(2). |
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| Authors: | Liao Xinbo Tang Weiping Zhao Yinghai Li Feihaong |
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| Abstract: | The structure and fine distribution of lymph capillaries were observed by using 5'-nu-cleotidase-Alkaline phosphatase (5'-Nase-Alpase) double staining method in the dermal of nude mouse human gastric carcinoma. Under light microscope lymph capillaries and vessels showed strong 5'-Nase positive reaction with brown or dark brown staining, while blood capillaries and vessels revealed significant ALPase activity with blue staining. On the basis of such characteristics, lymph and blood capillaries can be distinguished histochemically. Lymph capillaries, especially the solid line-like ones, can be demonstrated more objectively and accurately than using the routine H ?E staining method. |
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| Keywords: | Gastric carcinoma Skin of the nude mouse Lympn capillaries 5'-NaseALPase double staining method |
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