Crystallization and preliminary X-ray diffraction studies of a MAT alpha 2-DNA complex |
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Authors: | C Wolberger C O Pabo A K Vershon A D Johnson |
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Institution: | Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205. |
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Abstract: | Crystals have been obtained of the DNA-binding domain of the yeast MAT alpha 2 repressor bound to a 21 base-pair DNA site. The crystals are grown from polyethylene glycol and CaCl2 and form in space group P2(1) with a = 60.1 A, b = 39.4 A, c = 68.7 A and beta = 98 degrees. They diffract to 2.9 A resolution and contain one protein-DNA complex in the crystallographic asymmetric unit. |
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