Dermal bioactives from lactobacilli and bifidobacteria

Lactobacilli and bifidobacteria are the most common genera of probiotics with documented potentials on gut health. Recent studies have suggested that such potentials can be extended beyond gut well-being, such as that of dermal health. Our present study aimed to evaluate the production of bioactives that are essential for skin defense, such as lipoteichoic acid, peptidoglycan, hyaluronic acid, sphingomyelinase, lactic acid, acetic acid, and diacetyl, from lactobacilli and bifidobacteria grown in milk. All strains studied showed the presence of LTA in the cell wall fraction, with higher amounts from Lactobacillus rhamnosus FTDC 8313 and Bifidobacterium longum BL 8643 than other strains studied. Meanwhile, all strains studied showed equal concentrations of cell wall peptidoglycan. Our results showed that all strains studied were capable of producing hyaluronic acid, with higher production by lactobacilli than bifidobacteria. Production of diacetyl was more prevalent from strains of lactobacilli, while bifidobacteria produced higher amounts of acetic acid. Strains of lactobacilli and bifidobacteria studied also produced acid and neutral sphingomyelinase, an enzyme that generates ceramides and subsequent development of physical barriers in the stratum corneum. Our current findings show that bioactive and inhibitive extracts are produced from the fermentation of lactobacilli and bifidobacteria in milk, with potentials for dermal applications.