Viability of cryopreserved human skin allografts: effects of transport media and cryoprotectant |
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Authors: | Sonia Gaucher Caroline Elie Olivier Vérola Mohamed Jarraya |
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Institution: | (1) Facult? de M?decine, Universit? Paris Descartes, Paris, France;(2) Service des Br?l?s, AP-HP H?pital Cochin, 75014 Paris, France;(3) Service de Biostatistique, AP-HP H?pital Necker Enfants Malades, 75014 Paris, France;(4) Facult? de M?decine, Universit? Paris 7, Paris, France;(5) Service d’Anatomie Pathologie, AP-HP H?pital Saint Louis, 75010 Paris, France;(6) Banque des Tissus Humains, AP-HP H?pital Saint Louis, 75010 Paris, France |
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Abstract: | Human skin allografts can be preserved by different methods. In our clinical practice, human skin allografts are harvested
on multi-organ and tissue donors, transferred at +4°C in Ringer Lactate, cryopreserved with 15% Glycerol and held in the vapor
phase of a liquid nitrogen freezer until delivery to the burn center. The aim of this experimental study was to evaluate the
impact of transport medium and cryoprotectant on the viability of human skin allografts. For this purpose, we compared skin
samples harvested from 19 multi-organ and tissue donors with two different transport media and two different cryoprotectants.
Viability was assessed by the MTT assay after harvesting at laboratory reception, during storage (at +4°C) at day 2 and day
7, and after cryopreservation and thawing. Histopathological analysis was performed for each MTT assay. Results indicate that,
when stored at +4°C, skin retains more viability with RPMI, whereas Glycerol and DMSO are equivalent cryoprotectants regardless
of the transport medium. In conclusion, our protocol could be improved by the utilization of RPMI as transport medium. |
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