Preparation and characterization of catalase-positive particles ('microperoxisomes') from Harder's gland of the rat. |
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| Authors: | P Böck H Goldenberg M Hüttinger M Kolar R Kramar |
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| Institution: | Department of Micromorphology and Electron Microscopy, and Department of Medical Chemistry, University of Vienna, Vienna, Austria |
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| Abstract: | Catalase-positive particles (diameter 0.1–0.3 μm) from Harder's gland of the rat were prepared by differential centrifugation. It was demonstrated that these particles do not contain the oxidases thought to be characteristic of peroxisomal systems (i.e. urate oxidase, d-amino acid oxidase, and α-hydroxy acid oxidase). Cytochemical DAB reaction was employed to demonstrate the organelles in the gland tissue and in subcellular fractions by electron microscopy. |
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