Mutations in the <Emphasis Type="Italic">S</Emphasis> gene region of hepatitis B virus genotype <Emphasis Type="Italic">D</Emphasis> in Turkish patients |
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Authors: | Mehmet Özaslan Ersan Özaslan Arzu Barsgan Mehmet Koruk |
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Institution: | (1) Department of Biology, University of Gaziantep, 27310 Gaziantep, Turkey;(2) Department of Gastroenterology, Ankara Numune Education and Training Hospital, 06100 Ankara, Turkey;(3) Department of Gastroenterology, University of Gaziantep, 27310 Gaziantep, Turkey |
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Abstract: | The S gene region of the hepatitis B virus (HBV) is responsible for the expression of surface antigens and includes the ‘a’-determinant
region. Thus, mutation(s) in this region would afford HBV variants a distinct survival advantage, permitting the mutant virus
to escape from the immune system. The aim of this study was to search for mutations of the S gene region in different patient groups infected with genotype D variants of HBV, and to analyse the biological significance of these mutations. Moreover, we investigated S gene mutation inductance among family members. Forty HBV-DNA-positive patients were determined among 132 hepatitis B surface
antigen (HbsAg) carriers by the first stage of seminested PCR. Genotypes and subtypes were established by sequencing of the
amplified S gene regions. Variants were compared with original sequences of these serotypes, and mutations were identified.
All variants were designated as genotype D and subtype ayw3. Ten kinds of point mutations were identified within the S region. The highest rates of mutation were found in chronic hepatitis patients and their family members. The amino acid mutations
125 (M → T) and 127 (T → P) were found on the first loop of ‘a’-determinant. The other consequence was mutation inductance
in a family member. We found some mutations in the S gene region known to be stable and observed that some of these mutations
affected S gene expression. |
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Keywords: | Hepatitis B virus (HBV) hepatitis B surface antigen genotype D S gene region mutation |
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