Effects of temperature, storage period and the number of individuals on the detection of the false spider mite Cardinium endosymbiont |
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| Authors: | Valdenice M Novelli Juliana Freitas-Astúa Fernanda Arrivabem Eliane C Locali-Fabris Mark E Hilf Tim R Gottwald Marcos A Machado |
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| Institution: | (1) Laboratório de Biotecnologia, Centro APTA Citros “Sylvio Moreira”-IAC, Rodovia Anhanguera Km 158, P.O. Box 04, 13490-970 Cordeirópolis, SP, Brazil;(2) Embrapa Milho e Sorgo, Rod. MG 424, Km 65, CP 151, 35701-970 Sete Lagoas, MG, Brazil;(3) USDA-ARS-USHRL, Ft. Pierce, FL 34945, USA |
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| Abstract: | Cardinium have been found as endosymbionts of Brevipalpus phoenicis, the mite vector of the Citrus leprosis virus. With the long-term objective being to understand the mechanisms of plant-virus-vector interactions, we evaluated the different
storage conditions and periods, as well as the number of mites needed for PCR-amplification of such endosymbionts, making
it possible to collect mites in different geographical regions without prolonged storage compromising subsequent analyses. |
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| Keywords: | Brevipalpus phoenicis Sampling and conservation |
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