利用微卫星进行奶牛数量性状基因位点定位的研究

采用孙女设计法对德国奶牛的３个数量必状－－产奶量、乳脂产量及乳蛋白产量进行了数量性状基因位点的定位研究。实验共有２０个父系半同胞家系的１１３０头种公牛，共测定了３０对染色体上的２２９个微卫星，表型性状的育种值由孙代母牛估计得到。连锁分析采用多重遗传标记回归法，临界Ｆ值由置换实验法计算得到。统计发现在某些染色体上存在着一些显著的ＱＴＬ＇ｓ区域，特别是１４号染色体上某一区域对所有３个产奶性状都有极显著

Study of Mapping QTLs for Milk Traits on the German Dairy Cattle

A total of 1 130 bulls belonging to 20 half-sib families of German dairy cattle were genotyped for 229 microsatellite markers on 30 chromosomes. The data were used in an attempt to map quantitative trait loci applying regression as multiple-marker regression. For association analysis with a granddaughter design, the estimated breeding values for 3 milk traits were used: milk production, fat production and protein production. The empirical values of significance thresholds were determined by using a permutation test on the experimental data. Several significant QTLs were found on some chromosomes, especially on the chromosome 14. The results give a strong support for the experiments of Coppieters et al. and Ron et al.