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Non-invasive multi-species monitoring: real-time PCR detection of small mammal and squirrel prey DNA in pine marten (Martes martes) scats |
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| Authors: | Denise B. O’Meara Emma Sheehy Peter D. Turner Declan O’Mahony Andrew P. Harrington Huw Denman Colin Lawton Jenny MacPherson Catherine O’Reilly |
| Affiliation: | 1. Molecular Ecology Research Group, Waterford Institute of Technology, Cork Road, Waterford, Ireland 2. Mammal Ecology Group, School of Natural Sciences, Ryan Institute, National University of Ireland, Galway, Ireland 3. Ecological Management Group, Ormeau Business Park, Belfast, BT7 2JA, Northern Ireland, UK 4. Mammals in a Sustainable Environment (MISE) Project, Waterford County Council, Tramore Civic Offices, Tramore, Co., Waterford, Ireland 5. Selectfor, Brechfa, Carmarthenshire, Wales, UK 6. MISE Project, The Vincent Wildlife Trust, Brechfa, Carmarthenshire, Wales, UK |
| Abstract: | DNA identification of mammal species occurring in the diet of a predator is potentially a useful approach to remotely monitor the distribution of multiple species. This is important in Ireland, where it has been shown that the combined presence of the introduced bank vole and greater white-toothed shrew impact the distribution of the indigenous small mammals, the wood mouse and pygmy shrew. Direct monitoring of these species and their interactions requires trapping, a labour-intensive and costly approach. In this study, we applied an indirect method by genetically testing the presence of small mammals in pine marten scats collected during the National Pine Marten Survey (2005–2007) to map their distribution. We also included additional scats to investigate if less common prey items, the red squirrel and grey squirrel, could also be detected. This study demonstrates that all target species were genetically detected from pine marten scats. This strategy could be implemented as a monitoring programme for indigenous and introduced mammal species. |
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