Lycopene cyclization inBlakeslea trispora |
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Authors: | Bina J Mehta Enrique Cerdá-Olmedo |
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Institution: | (1) Departamento de Genética, Universidad de Sevilla, Apartado 1095, E-41080 Sevilla, Spain |
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Abstract: | Fungi produce and accumulate various carotenoids. Mycelia of the ZygomyceteBlakeslea trispora contained β-carotene and its precursors γ-carotene and lycopene. When strains of opposite sex grew together, the β-carotene
concentration increased fourfold, that of γ-carotene remained unchanged, and other intermediates practically disappeared.
The inhibitors nicotine, 2-(4-chlorophenylthio)-triethylamine, α-picoline, and imidazole increased the concentrations of lycopene
and γ-carotene and decreased those of β-carotene. From our quantitative results, we conclude thatBlakeslea has two pathways for lycopene metabolism, of which other fungi have only one or the other. The main one, two cyclizations
from lycopene to β-carotene, is carried out by an enzyme dimer, is stimulated by sexual interaction, and is sensitive to the
inhibitors. The other pathway, a cyclization to γ-carotene is not affected by mating or the inhibitors. |
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Keywords: | Blakeslea trispora carotene cyclase inhibitors lycopene cyclization sexual activation |
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