Modulation of DNA intercalation by resveratrol and genistein |
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Authors: | Subbiah Usha Irudayam Maria Johnson Raghunathan Malathi |
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Institution: | (1) Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, 600 113, India;(2) Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center, 874 Union Avenue, Memphis, Tennessee, 38163, USA |
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Abstract: | Time correlated Single Photon Counting study (TCSPC) was performed for the first time to evaluate the effect of resveratrol
(RES) and genistein (GEN) at 10–100 μM and 10–150 μM respectively, in modulating the DNA conformation and the variation induced
due to intercalation by the dyes, ethidium bromide (EtBr) and acridine orange (AO). It is demonstrated using UV-absorption
and fluorescence spectroscopy that RES and GEN, at 50 μM and 100 μM respectively can bind to DNA resulting in significant
de-intercalation of the dyes, preventing their further intercalation within DNA. Hyperchromicity with red/blue shifts in DNA
when bound to dyes was reduced upon addition of RES and GEN. DNA-dependent fluorescence of EtBr and AO was quenched in the
presence of RES by 87.97% and 79.13% respectively, while similar quenching effect was observed for these when interacted with
GEN (85.52% and 83.85%). It is found from TCSPC analysis that the higher lifetime component or constituent of intercalated
dyes (τ2, A
2) decreased with the subsequent increase in smaller component or constituent of free dye (τ1, A
1) after the interaction of drugs with the intercalated DNA. Thus these findings signify that RES and GEN can play an important
role in modulating DNA intercalation, leading to the reduction in DNA-directed toxicity. |
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Keywords: | resveratrol genistein ethidium bromide acridine orange DNA TCSPC analysis |
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