Identification of a set of RFLP probes for subspecies differentiation in Oryza sativa L. |
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Authors: | H-R Qian J-Y Zhuang H-X Lin J Lu K-L Zheng |
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Institution: | (1) Biotechnology Department, China National Rice Research Institute, 310006 Hangzhou, P. R. China |
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Abstract: | Sixty-eight indica-japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Oryza sativa L.) with four enzyme digestions (EcoRI, EcoRV, HindIII and DraI). Twenty-one DNA clones were isolated as indica-japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica-japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L. |
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Keywords: | Restriction fragment length polymorphism (RFLP) Probe screening Subspecies differentiation Wide compatibility variety Oryza sativa L |
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