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A simple in-vitro 'wet-plate' method for mass production of Phytophthora nicotianae zoospores and factors influencing zoospore production |
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| Authors: | Ahonsi Monday O Banko Thomas J Hong Chuanxue |
| Affiliation: | aHampton Roads Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, 1444 Diamond Springs Road, Virginia Beach, VA 23455, USA |
| Abstract: | A simple in-vitro ‘wet-plate’ method for mass-producing Phytophthora nicotianae zoospores at ≥ 1.0 × 106 zoospores/ml is described. Temperature critically affected zoospore production; 22 °C was optimum, while 36 °C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops. |
| Keywords: | Phytophthora Recycled irrigation water Zoospore production |
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