| 聚球藻7002在光生物反应器中的光自养培养 |
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| 引用本文： | 施定基.聚球藻7002在光生物反应器中的光自养培养[J].生物工程学报,2000,16(5):618-622. |
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| 作者姓名： | 施定基 |
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| 作者单位： | 1. 中国科学院化工冶金研究所,生化工程国家重点实验室,北京,100080
2. 中国科学院植物研究所,北京,100093 |
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| 摘 要： | 通过对聚球藻7002在光生物反应器中的培养，研究了光强在聚球藻7002培养液中的衰减规律，得到了培养过程光强随藻细胞浓度和光程距离变化的关系式，即I=I0exp［-(-0.0239+0.0777OD750)·L］。并对培养过程特性及培养温度、外加CO2浓度和光照强度对藻细胞生长的影响进行了较为详细的研究，得到了反应器中较为适宜的聚球藻7002的培养条件，藻细胞培养密度达到3.4g/L(干重)，体积产率达到0.57g/(L·d)的较高水平。
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| 关 键 词： | 聚球藻7002，光生物反应器，光自养培养，光衰减 |
| 文章编号： | 1000-3061(2000)05-0618-05 |
| 修稿时间： | 1999-11-16 |
Photoautotrophic Cultivation of Synechococcus sp. PCC7002 in Photobioreactor |
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| KANG Rui-Juan,ZHOU Wen-Qi,CAI Zhao-Ling,SHI Ding-Ji.Photoautotrophic Cultivation of Synechococcus sp. PCC7002 in Photobioreactor[J].Chinese Journal of Biotechnology,2000,16(5):618-622. |
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| Authors: | KANG Rui-Juan ZHOU Wen-Qi CAI Zhao-Ling SHI Ding-Ji |
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| Institution: | KANG Rui-Juan
,ZHOU Wen-Qi
,CAI Zhao-Ling
;(State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, The Chinese Academy of Sciences, Beijing 100080);SHI Ding-Ji
;(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093) |
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| Abstract: | The Photoautotrophic cultivation of Synechococcus sp. PCC 7002 in a 15 L-airlift photobioreactor was carried out. The changes of light intensity with cell density and optical length in the cultivation system were investigated. Based on experimental results, the light attenuation could be described by I = I0exp -(-0.0239 + 0.0777 OD750).L]. The effects of the variations of light intensity, CO2 concentration in gas inlet and culture temperature on the growth of cells during the cultivation process have also been studied. The optimized condition was determined and a high dry cell density of 3.4 g/L was obtained. The volumetric productivity reached 0.57 g/(L.d) under the optimized condition. |
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| Keywords: | Synechococcus sp PCC7002 photobioreactor photoautotrophic cultivation light intensity attenuation |
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