Supercooling,ice nucleation and crystal growth: a systematic study in plant samples |
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| Affiliation: | 1. Department of Forestry and Management of Natural Environment, Technological Educational Institute (TEI) of Thessaly, 413 35 Larissa, Greece;2. Department of Computer Engineering, Technological Educational Institute (TEI) of Thessaly, 413 35 Larissa, Greece;3. Department of Plant Production, Technological Educational Institute (TEI) of Thessaly, 413 35 Larissa, Greece;1. Department of Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark;2. Department of Science and Environment, Roskilde University, Universitetsvej 1, 4000 Roskilde, Denmark;1. Pharmaceutical and Heterocyclic Compounds Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran;2. Polyurethane Department, Iran Polymer and Petrochemical Institute, P. O. Box 14965-115, Tehran, Iran;3. Present address: Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran |
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| Abstract: | This paper presents an innovative technological platform which is based on infrared video recording and is used for monitoring multiple ice nucleation events and their interactions, as they happen in 96 well microplates. Thousands of freezing curves were obtained during this study and the following freezing parameters were measured: cooling rate, nucleation point, freezing point, solidus point, degree of supercooling, duration of dendritic phase and duration of crystal growth. We demonstrate the use of this platform in the detection of ice nuclei in plant samples. Future applications of this platform may include breeding for frost tolerance, cryopreservation, frozen food technology and atmospheric sciences. |
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| Keywords: | Supercooling Latent heat Ice nucleation Freezing High throughput screening Sea buckthorn Thermal image processing Grape |
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