Divergent forms of endoplasmic reticulum stress trigger a robust unfolded protein response in honey bees |
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| Affiliation: | 1. Biology Department, Barnard College, New York, NY 10027, USA;2. Faculty of Life Sciences, University of Manchester, Manchester, UK;3. U1053 INSERM, Université de Bordeaux, France;4. Biology Department, The City College of New York – CUNY, New York, NY 10031, USA;1. Biologie I, University of Regensburg, Universitätsstraße 31, D-93040 Regensburg, Germany;2. Institute of Functional Genomics, University of Regensburg, Josef-Engert-Str. 9, D-93051 Regensburg, Germany;1. The H. Niewodniczański Institute of Nuclear Physics Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland;2. Research Center for Structural Thermodynamics, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan;3. Department of Chemistry, Graduate School of Science, Osaka University, Machikaneyama 1-1, Toyonaka, Osaka 560-0043, Japan;4. Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland;1. Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, 05508-900 São Paulo, SP, Brazil;2. Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-909 Rio de Janeiro, RJ, Brazil;1. Institut de Recherche sur la Biologie de l’Insecte, UMR 7261, CNRS/Université François-Rabelais de Tours, Tours, France;2. Laboratoire Biologie Végétale et Biomolécules, EA 2106, Université François-Rabelais de Tours, Tours, France;3. Department of Biochemistry, Max-Planck-Institute for Chemical Ecology, Jena, Germany;4. Institut de Recherche en Horticulture et Semences, UMR 1345, INRA, SFR 4207 QuaSaV, Beaucouzé, France |
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| Abstract: | Honey bee colonies in the United States have suffered from an increased rate of die-off in recent years, stemming from a complex set of interacting stresses that remain poorly described. While we have some understanding of the physiological stress responses in the honey bee, our molecular understanding of honey bee cellular stress responses is incomplete. Thus, we sought to identify and began functional characterization of the components of the UPR in honey bees. The IRE1-dependent splicing of the mRNA for the transcription factor Xbp1, leading to translation of an isoform with more transactivation potential, represents the most conserved of the UPR pathways. Honey bees and other Apoidea possess unique features in the Xbp1 mRNA splice site, which we reasoned could have functional consequences for the IRE1 pathway. However, we find robust induction of target genes upon UPR stimulation. In addition, the IRE1 pathway activation, as assessed by splicing of Xbp1 mRNA upon UPR, is conserved. By providing foundational knowledge about the UPR in the honey bee and the relative sensitivity of this species to divergent stresses, this work stands to improve our understanding of the mechanistic underpinnings of honey bee health and disease. |
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| Keywords: | Cellular stress response Proteostasis Endoplasmic reticulum Unfolded protein response IRE1 Xbp1 Honey bee |
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