Optimization of wrMTrck to monitor Drosophila larval locomotor activity |
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| Institution: | 1. Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany;2. German Center for Neurodegenerative Diseases (DZNE), D-37077 Göttingen, Germany;3. Dept. Molecular Microbiology and Genetics, Institute of Microbiology & Genetics, Georg-August-Universität Göttingen, D-37077 Göttingen, Germany;4. Department of Neurodegeneration and Restorative Research, University Medicine Göttingen, Göttingen, Germany;5. DFG Research Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, Germany;6. Max Planck for Structural Biology, Chemistry and Molecular Biophysics of Rosario (MPLbioR), Universidad Nacional de Rosario, IBR-CONICET, Ocampo y Esmeralda, 2000 Rosario, Argentina;7. Center for Integrated Protein Science Munich, Chemistry Department, Technische Universität München, Lichtenbergstr. 4, 85748 Garching, Germany;8. Max-Planck-Institute of Molecular Physiology, Department of Physical Biochemistry, Dortmund 44227, Germany;9. Department of Genetics, University of Leicester, Leicester LE1 7RH, UK;1. Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA;2. ICF International, Durham, NC, USA |
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| Abstract: | An efficient and low-cost method of examining larval movement in Drosophila melanogaster is needed to study how mutations and/or alterations in the muscular, neural, and olfactory systems affect locomotor behavior. Here, we describe the implementation of wrMTrck, a freely available ImageJ plugin originally developed for examining multiple behavioral parameters in the nematode C. elegans. Our optimized method is rapid, reproducible and does not require automated microscope setups or the purchase of proprietary software. To demonstrate the utility of this method, we analyzed the velocity and crawling paths of two Drosophila mutants that affect muscle structure and/or function. Additionally, we show that this approach is useful for tracking the behavior of adult insects, including Tribolium castaneum and Drosophila melanogaster. |
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| Keywords: | Larval locomotion Behavior assay Muscle |
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