Effect of sericin supplementation in maturation medium on cumulus cell expansion,oocyte nuclear maturation,and subsequent embryo development in Sanjabi ewes during the breeding season |
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| Institution: | 1. Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, PR China;2. Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, PR China;1. INRA, Physiologie de la Reproduction et des Comportements, Nouzilly, France;2. Faculty of Veterinary, Laboratory of Physiology and Control of Reproduction (LFCR), State University of Ceará, Fortaleza, Ceara, Brazil;3. Museum National d''Histoire Naturelle, Réserve de la Haute Touche, Obterre, France;4. Faculty of Veterinary, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceara, Brazil;1. Cátedra de Teriogenología, Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, Corrientes, Argentina;2. Facultad de Ingeniería y Ciencias Agrarias, Pontificia Universidad Católica Argentina, Buenos Aires, Argentina;3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina;1. State Key Laboratory of Animal Nutrition, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, People''s Republic of China;2. Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas;1. ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India;2. ICAR-National Dairy Research Institute, Karnal, Haryana, India |
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| Abstract: | The purpose of this study was to evaluate the effect of sericin with different concentrations (0% control], 0.1%, 0.5%, 1.0%, and 2.5%) added to the IVM medium on cumulus cell expansion, oocyte nuclear maturation, and subsequent embryo development in Sanjabi ewes during the breeding season. The resumption of meiosis was assessed by the frequency of germinal vesicle breakdown and the first polar body extrusion. After IVF with fresh ram semen, presumptive zygotes were cultured 8 days in potassium simplex optimization medium supplemented by amino acids, and the percentages developing to the two-cell and blastocyst stages were measured as the indicators of early embryonic developmental competence. More cumulus–oocyte complexes matured with 0.5% sericin underwent germinal vesicle breakdown and reached metaphase II stage compared with the control cumulus–oocyte complexes matured without sericin (P ≤ 0.05). The present findings indicated that supplementation with 0.5% sericin during the maturation culture may improve the nuclear maturation and the cumulus cell expansion. Furthermore, the percentage of blastocysts obtained from 0.5% and 0.1% sericin (37.8 ± 1.76% and 34.8 ± 1.09%, respectively) was higher (P ≤ 0.05) than that of the control medium (29.60 ± 1.67%). However, addition of 1% and 2.5% of sericin to the IVM medium oocytes had a negative effect on nuclear maturation and cumulus cell expansion. Furthermore, the percentage of cleavage and blastocyst rate was significantly lower in the 1% and 2.5% sericin groups than in the control group. These findings showed that supplementation of IVM medium with 0.5% sericin may improve the meiotic competence of oocytes and early embryonic development in Sanjabi ewes during the breeding season. |
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| Keywords: | Ovine Cumulus expansion IVM Sericin Embryo development |
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