反义凝血酶受体基因的表达对人ASMC增殖的影响

介入治疗后再狭窄的发生严重降低了该治疗手段的最终疗效．为探讨再狭窄的发生机理、寻找有效的预防手段，利用反义ＲＮＡ技术构建了含有部分反义凝血酶受体（ＡＴＲ）ｃＤＮＡ片段的真核表达质粒ｐｃＤＮＡ３／ＡＴＲ，并观察了其对培养的人主动脉平滑肌细胞（ＡＳＭＣ）增殖的影响．结果表明ｐｃＤＮＡ３／ＡＴＲ的瞬时转染即能显著抑制人ＡＳＭＣ的３Ｈ－ＴｄＲ参入量，且该作用与导入的ＤＮＡ量呈剂量依赖性．说明部分反义凝血酶受体基因的表达可以抑制ＡＳＭＣ的增殖

Effect of Antisense Thrombin Receptor Gene on the Proliferation of Human Vascular Smooth Muscle Cells

Restenosis after angioplasty severely limits the final effect of this therapeutic technique. To study the fundamental mechanisms of restenosis and search for an effectively preventive approach, a eukaryotic expression vector (pcDNA3/ATR) containing a partial antisense thrombin receptor (ATR) gene was constructed by antisense RNA technique. The inhibition of the recombinant vector proliferation of cultured human aortic smooth muscle cells (ASMC) was studied after introducing pcDNA3/ATR into ASMC instantaneously. The results showed that 3 H TdR incorporation in the transfected human ASMC was inhibited with pcDNA3/ATR in a dose dependent manner. The expression of a partical antisense thrombin receptor gene could inhibit the proliferation of human ASMC.