The Organization of rRNA Genes in Vicia faba |
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Authors: | Yakura Kimitaka; Tanifuji Shigeyuki |
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Institution: | Department of Botany, Faculty of Science, Hokkaido University Sapporo 060, Japan |
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Abstract: | Vicia faba DNA was digested with restriction endonucleases andfractionated on 1% agarose gels. The physical map for EcoRI,BamHI and XbaI cleavage sites in V. faba cytosol rDNA was determinedusing the Southern blot hybridization technique. XbaI and BglIIdigestion or partial EcoRI digestion showed that the lengthof a major repeat unit of V. faba rDNA is 6.7 x 106 daltons.EcoRI and BamHI cleaved this unit into two and five DNA fragments,respectively. (Received April 23, 1981; Accepted July 20, 1981) |
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