Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry |
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Authors: | Neora?Pick Scott?Cameron Dorit?Arad Email author" target="_blank">Yossef?Av-GayEmail author |
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Institution: | (1) Department of Medicine, Division of Infectious Diseases, University of British Columbia, 2733 Heather Street, D450 HP East, V5Z 3J5 Vancouver, BC, Canada |
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Abstract: | The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to
enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound’s toxicity is
essential in the development of novel agents. Our interest is in a rapid, simple, high throughput screening method to assess
cytotoxicity induced by potential agents. Some intracellular pathogens, such as Mycobacterium tuberculosis primary site of infection is human alveolar macrophages. Thus, evaluation of candidate drugs for macrophage toxicity is crucial.
Protocols for high throughput drug toxicity screening of macrophages using flow cytometry are lacking in the literature. For
this application we modified a preexisting technique, propidium iodide (PI) exclusion staining and utilized it for rapid toxicity
tests. Samples were prepared in 96 well plates and analyzed by flow cytometry, which allowed for rapid, inexpensive and precise
assessment of compound’s toxicity associated with cell death.
Published: October 1, 2004. |
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Keywords: | Indexing terms" target="_blank">Indexing terms Flow Cytometry Propidium Lethal Dose 50 |
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