Characterisation of coliphage K30, a bacteriophage specific for Escherichia coli capsular serotype K30 |
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Authors: | Christopher Whitfield Michele Lam |
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Institution: | Department of Microbiology, College of Biological Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada |
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Abstract: | Abstract Coliphage K30, a bacteriophage specific for strains bearing the Escherichia coli serotype K30 capsular polysaccharide, produced plaques surrounded by extensive haloes, a characteristic of phage which produce capsule depolymerase (glycanase) enzymes. Klebsiella K20, a strain producing a capsular polysaccharide chemically identical to that of E. coli K30, was not lysed by coliphage K30, although the bacteriophage encoded glycanase enzyme did degrade the K20 polysaccharide. Morphologically, coliphage K30 belonged to Bradley group C. The coliphage K30 particle comprised 20 structural polypeptides which varied from 9.5–136 kDa and genomic DNA of 38.7 ± 1.0 kb. |
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Keywords: | Escherichia coli bacteriophage capsular polysaccharide depolymerase enzyme |
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