Detection and partial characterization of a bacteriocin produced by Carnobacterium piscicola 213 |
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Authors: | Z Khouiti J P Simon |
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Institution: | (1) Service de Microbiologie, Faculté des Sciences, Université Libre de Bruxelles, Belgium, BE;(2) Unité de BioTechnologie, Institut Meurice, Ceria, 1 avenue Emile Gryzon, B-1070 Bruxelles, Belgium, BE |
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Abstract: | BLIS 213, is a bacteriocin-like inhibitory substance produced by Carnobacterium piscicola 213. It is active against Carnobacterium, Enterococcus and Listeria spp. No activity was observed against tested Lactobacillus, Lactococcus, Leuconostoc and Pediococcus strains, nor against Gram-negative bacteria. The BLIS 213 activity was inactivated by several proteolytic enzymes. It was
heat resistant (121°C for 20 min), and stable over a pH range of 2–8. Activity was determined by a dilution micromethod;
it was increased after SDS treatment. A mutant strain which lacks bacteriocin production was isolated and designated as
Carnobacterium piscicola 213a. It had the same phenotypic and biochemical properties as the parent strain, and was not sensitive to bacteriocin activity.
The apparent molecular weight of the bacteriocin in the crude extract was greater than 10 kDa. It was about 6 kDa after
SDS-PAGE of a partially purified bacteriocin by adsorption on producer cells. The isoelectric point of the BLIS 213 was around
9.3.
Received 21 January 1997/ Accepted in revised form 25 April 1997 |
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Keywords: | : lactic acid bacteria Carnobacteria bacteriocins |
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