Restricted expression of LUZP in neural lineage cells: A study in embryonic stem cells |
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Authors: | Maud Wan-Ying Lee Alice Chien Chang Der-Shan Sun Chia-Yi Hsu Nan-Chi A. Chang PhD |
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Affiliation: | Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan, ROC. |
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Abstract: | A novel protein LUZP with 3 leucine zipper motifs at its amino terminus is predominantly expressed in the adult brain. A modified gene targeting approach was employed in an attempt to establish in vitro and in vivo models in which Luzp is knock-out (KO) for phenotype assessment and a reporter gene lacZ is knock-in (KI) for tracing its expression. We report in this study the molecular cloning of the Luzp gene, its targeting vector construction and Luzp-KO/lacZ-KI embryonic stem (ES) clone selection. Since LUZP is also expressed in ES cells, the possibility of embryonic lethality cannot be excluded when attempting to establish Luzp-null mutant mice. We have therefore examined the development of homozygous Luzp-KO/lacZ-KI clones in nude mice. Tissue types derived from all three embryonic germ layers were observed in teratomas developed in nude mice. In situ X-gal staining further revealed restricted expression of LUZP in neural lineage cells. |
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Keywords: | Leucine zipper Embryonic stem cell Neural lineage |
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