The Mouse Spam1 maps to proximal Chromosome 6 and is a candidate for the sperm dysfunction in Rb(6.16)24Lub and Rb(6.15)lAld heterozygotes |
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| Authors: | Xiangning Deng Jennifer Moran Neal G Copeland Debra J Gilbert Nancy A Jenkins Paul Primakoff Patricia A Martin-DeLeon |
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| Institution: | (1) Department of Biology, University of Delaware, Newark, Delaware 19716-2590, USA, US;(2) Molecular Biology Department, Princeton University, Princeton, New Jersey, USA, US;(3) Mammalian Genetics Laboratory, ABL-Basic Research Program, Frederick Cancer Research Center, P.O. Box B, Frederick, Maryland, USA, US;(4) Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA, US |
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| Abstract: | We have determined the chromosomal localization of the murine gene encoding the 68-kDa sperm adhesion molecule 1, Spam1 or Ph-20. Using two independent approaches, fluorescence in situ hybridization (FISH) and interspecific backcross analysis, we show
that Spam1 maps to proximal mouse Chromosome (Chr) 6. This map position is within the conserved linkage group corresponding to human
Chr 7q, where the human homolog, SPAM 1, has been shown to map previously. Genetic mapping shows the gene to be very closely
linked to Met, one of the most proximal loci on MMU 6. It thus places the gene near the centromere and the junction of the Rb(6.16)24Lub
and Rb(6.15)1Ald translocations. The essential role of the Spam1 sperm antigen in mouse sperm-egg interactions and its gene
location provide strong support for its candidacy as the gene involved in the dysfunction of mouse sperm bearing the Rb(6.16)24Lub
or Rb(6.15)1Ald translocation.
Received: 16 July 1996 / Accepted: 23 September 1996 |
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