Extracellular ATP itself elicits superoxide generation in guinea pig peritoneal macrophages. |
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| Authors: | M Nakanishi H Takihara Y Minoru K Yagawa |
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| Institution: | Second Department of Internal Medicine, Faculty of Medicine, Fukuoka University, Japan. |
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| Abstract: | Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium (Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of Ca2+]i mobilization. Pre-exposure to a small amount of phorbol myristate acetate (PMA) primed the ATP-induced generation of O2- without a change of Ca2+]i. The results suggest that ATP-induced O2- generation is mediated by Ca2+]i mobilization and by PT-sensitive G protein. |
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