| 红豆杉细胞悬浮培养结构化数学模型的探讨 |
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| 引用本文： | 梅兴国,余斐,张姝,汤志群. 红豆杉细胞悬浮培养结构化数学模型的探讨[J]. 生物技术, 2000, 10(3): 8-11 |
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| 作者姓名： | 梅兴国 余斐 张姝 汤志群 |
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| 作者单位： | 华中理工大学生命科学与技术学院,湖北,武汉,430074 |
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| 基金项目： | 国家“九五”科技攻关重点项目!(96 -C0 2 -0 3- 0 1) |
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| 摘 要： | 用１０Ｌ机械搅拌式生物反应器悬浮培养红豆杉细胞，得到细胞生长、基质消耗和紫杉醇合成动力学曲线。经过代谢动力学分析建立了结构化数学模型。并将模型值与实验值进行比较，结果表明模型预测值与实验值较吻合。
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| 关 键 词： | 红豆杉细胞 悬浮培养 紫杉醇 结构化数学模型 |
Research of structured kinetic model for Taxus chinensis cell suspension culture |
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| MEI Xing-guo,YU Fei,ZHANG Shu,TANG Zhi-qun. Research of structured kinetic model for Taxus chinensis cell suspension culture[J]. Biotechnology, 2000, 10(3): 8-11 |
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| Authors: | MEI Xing-guo YU Fei ZHANG Shu TANG Zhi-qun |
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| Abstract: | The growth of plant cell cultures of Taxus chinensis in a ten Liter scale batch bioreactor was studied. On the basis of the existing knowledge with respect to secondary metabolism in cultured Taxus chinensis cells, a structured mathematical model was developed. Parameters for kinetic rate expressions within the model were estimated based on experimental data. Using these parameters, culture growth, substrate uptake, and taxol production were all successfully correlated to experimental data from batch cultures of Taxus chinensis. |
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| Keywords: | Taxus chinensis cell suspension culture Taxol structured kinetic |
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