Autoimmunity to glial antigens in vitro. |
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Authors: | T. Wahlström Ingegerd Hellström K.E. Hellström |
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Affiliation: | 1. Division of Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA;2. University of Washington School of Medicine, Seattle, Washington 98195 USA |
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Abstract: | Lymph nodes cells and spleen cells in a 50:50 mixture from Fischer 344 rats were cultured on syngeneic glial cells and fibroblasts. In the glia cultures, but not in the fibroblast cultures, the lymphocytes were stimulated to a vivid blast transformation and mitotic activity with a peak after 7 days, after which they reverted to small- and medium-sized lymphocytes. The stimulated lymphoid cells were not cytotoxic to glial cells when tested in a microcytotoxicity assay. A fraction of the lymphoblasts and their progeny (approximately 4%) took a positive intracytoplasmic stain for γ-globulin immunoglobulin G after direct immunofluorescence. Efforts to demonstrate immunoglobulin produced and released into the culture medium by the stimulated cells were negative. The findings may indicate that among the lymphoid cells responding to the glial antigens under these conditions, there are suppressor cells that abrogate the killer cell effect. |
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Keywords: | PFC plaque-forming cells SRBC sheep erythrocytes HBSS Hank's balanced salt solution BSA bovine serum albumin PWM pokeweed mitogen TT tetanus toxoid LMF-PWM lymphocyte mitogenic factor produced with PWM LMF-TT lymphocyte mitogenic factor produced with TT cpm counts per minute NRS normal rabbit serum RAG rabbit anti-human IgG |
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