Mobile phase compositions for ceramide III by normal phase high performance liquid chromatography |
| |
| Authors: | Seung?Pyo?Hong Chong?Ho?Lee Se?Kyung?Kim Hyun?Shik?Yun Jung?Heon?Lee Email author" target="_blank">Kyung?Ho?RowEmail author |
| |
| Institution: | (1) Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 402-751 Incheon, Korea;(2) Department of Biological Engineering, Inha University, 402-751 Incheon, Korea;(3) Department of Chemical Engineering, Chosun University, 501-759 Kwangju, Korea |
| |
| Abstract: | Ceramide III was prepared by the cultivation ofSaccharomyces cerevisiae. Ceramide III was partitioned from the cell extracts by solvent extraction and analyzed by Normal Phase High Performance
Liquid Chromatography (NP-HPLC) using Evaporative Light Scattering Detector (ELSD). We experimentally determined the mobile
phase composition to separate ceramide III with NP-HPLC. Three binary mobile phases of n-hexane/ethanol,n-hexane/Isoprophyl Alcohol (IPA) andn-hexane/n-butanol and one ternary mobile phase ofn-hexane/IPA/methanol were demonstrated. For the binary mobile phase ofn-hexane/ethanol, the first mobile phase composition, 95/5 (v/v), was step-increased to 72/23 (v/v) at 3 min. In the binary
mobile phase, the retention time of ceramide III was 7.87 min, while it was 4.11 min respectively in the ternary system, where
the mobile phase composition ofn-hexane/IPA/methanol, 85/7/8 (v/v/v), was step-increased to 75/10/15 (v/v/v) at 3 min. However, in the ternary mobile phase,
the more peak area of ceramide III was observed. |
| |
| Keywords: | Saccharomyces cerevisiae cermaide III mobile phase NP-HPLC ELSD |
| 本文献已被 SpringerLink 等数据库收录！ |
|
