Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants |
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Authors: | Arie Rosner and Ezra Yagil |
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Affiliation: | (1) Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel |
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Abstract: | Summary The effect of deoxyadenosine (AdR) and 5-fluorodeoxyuridine (FUdR) on the incorporation of 5-bromodeoxyuridine (BUdR) into DNA of thymine-non requiring cells of Escherichia coli was studied. This incorporation renders the cells sensitive to irradiation at near-UV light. It was found that the combination of AdR and FUdR increases incorporation of BUdR and sensitivity to irradiation.An enrichment of auxotrophs by a factor of 105 was obtained from a mixed population grown in minimal medium containing BUdR, AdR and FUdR and irradiated subsequently.Part of an M.Sc. Thesis in Microbiology submitted by A. Rosner to the Tel-Aviv University. |
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