Determination of epitestosterone and testosterone in urine by high-performance liquid chromatography |
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Authors: | R. Navajas, C. Imaz, D. Carreras, M. Garcí a, M. P rez, C. Rodrí guez, A. F. Rodrí guez,R. Cort s |
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Affiliation: | R. Navajas, C. Imaz, D. Carreras, M. García, M. Pérez, C. Rodríguez, A. F. Rodríguez,R. Cortés |
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Abstract: | This paper describes an alternative HPLC method for the determination of testosterone and epitestosterone, which is simple, rapid, selective, sensitive and reproducible. Samples were prepared using a previous enzymatic hydrolysis with liquid-liquid extraction. The determination was carried out on a Hypersil BDS-C18 reversed-phase column with gradient elution and UV absorbance detection (240 nm). The limits of quantification (signal-to-noise ratio = 6) were 20 ng/ml for testosterone and 30 ng/ml for epitestosterone. |
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