Antigen-binding receptors on T cells from long-term MLR. Evidence of binding sites for allogeneic and self-MHC products |
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Authors: | Bruce E Elliott Zoltan A Nagy Bela J Takacs Yinon Ben-Neriah David Givol |
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Institution: | (1) Cancer Research Laboratories, Botterell Hall, Department of Pathology, Queen's University, K7L 3N6 Kingston, Ontario, Canada;(2) Department of F/Konzern/PAI, F. Hoffman-LaRoche and Company, CH-4002 Basel, Switzerland;(3) Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel;(4) Present address: Department of Immunogenetics, Max Planck Institute for Biology, 7400 Tübingen 1, Germany |
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Abstract: | Antibody inhibition of radiolabelled stimulator membrane vesicle binding by T blasts activated in the mixed lymphocyte reaction (MLR) was used to identify responder-cell determinants involved in the binding phenomenon. Antisera or monoclonal antibodies against Thy-1, Lyt-1, Lyt-2 and Ly-6 antigens were not inhibitory. However, antibodies against heavy-chain V region (VH) determinants strongly inhibited vesicle binding by both primary and longterm MLR blasts. Anti-Ia (both alloantisera and monoclonal reagents) caused inhibition of antigen binding by primary MLR blasts only. T blasts from long-term MLR lines were neither Ia-positive, nor susceptible to blocking of antigen binding with anti-Ia. However, these cells were capable of specifically absorbing soluble syngeneic Ia material, with the concomitant appearance of vesiclebinding inhibition with anti-Ia sera. Acquisition of syngeneic Ia by T blasts was effectively blocked with the anti-VH reagent. Passively bound self-Ia did not interfere with vesicle binding in the absence of anti-Ia. These results strongly suggest the existance of specific self-Ia acceptor sites closely linked to the receptors for stimulator alloantigens on T cells proliferating in MLR. A receptor model based on these findings is briefly discussed.Abbreviations used in this paper B10
C57BL/10
- Con A
concanavalin A
- FcR
Fc receptor
- FCS
fetal calf serum
- H
heavy chain
- Ia
I-region associated antigen
- Ig
immunoglobulin
- LPS
lipopolysaccharide
- Lyt
T-lymphocyte differentiation antigen
- MHC
major histocompatibility complex
- MLR
mixed lymphocyte reaction
- PM
plasma membrane
- T
thymus derived
- Tcr
T-cell receptor
- V
variable region of Ig |
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