Physical studies of rabbit muscle phosphofructokinase: sedimentation behavior of the enzymatic reactive form. |
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| Authors: | L K Hesterberg J C Lee |
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| Affiliation: | The E. A. Doisy Department of Biochemistry, St. Louis University School of Medicine, 1402 South Grand Boulevard, St. Louis, Missouri 63104 U.S.A. |
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| Abstract: | Reacting enzyme sedimentation experiments were carried out to identify the active form of rabbit muscle phosphofructokinase in glycylglycine buffer at pH 8.55 and 23 ± 1 °C. Results from these experiments reveal one active form with s20,w = 12.2 ± 0.3 and 12.2 ± 0.3 S in a coupled enzyme and a pH-dependent dye linked system, respectively. |
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