Control of basidiospore production by a nuclear gene in the fungus Coprinus congregatus |
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Authors: | Y Bastouill-Descollonges G Manachère |
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Institution: | (1) Laboratoire de Mycologie, Université Lyon I, U.A. C.N.R.S., 1127, Bât. 405, 43 Boulevard du 11 Novembre 1918, F-69 622 Villeurbanne Cedex, France |
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Abstract: | Summary The genetical control of basidiospore production by sporophores of the fungus Coprinus congregatus was studied. This species is characterized by a bipolar compatibility control, and homokaryons with complementary alleles A1 and A2 can be distinguished apart. We confirmed that the pale mushroom phenotype of the fungus is determined by a nuclear gene symbolized pal. This gene also controls a sporeless character and segregates independently of the mating-type locus. Dikaryons homoallelic for the pal
– allele produce typical pale and sporeless sporophores, while heteroallelic (pal
+, pal
–) and homoallelic (pal
+, pal
+) dikaryons produce normal or almost normal sporulating sporophores. In order to segregate homokaryons homoallelic for the pal gene (A1, pal
–; A1, pal
+, A2, pal
–; A2, pal
+), the following protocols were used: (a) the dikaryotization of stock homokaryons containing the pal
+ allele and of each mating type, A1 or A2, by dikaryotic mycelia homoallelic for the pal
– allele; (b) the culturing of homokaryotic mycelia issuing from the germination of basidiospores from sporophores produced by dikaryotic mycelia heterokaryotic for the pal gene; (c) the culturing of mycelia grown from protoplasts obtained from dikaryons homoallelic for the pal
– allele (D6 strain), and from homokaryons heteroallelic for the pal gene (H8), or homoallelic for pal
#x002B;+ allele (H7). These techniques enabled us to segregate homokaryons of the four types defined above and were indispensable in the segregation of the pal
– homoallelic homokaryons as no basidiospores were produced by typical pale mushrooms. |
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Keywords: | Sporogenesis gene control Fruiting fungi Coprinus congregatus |
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