Synthesis of eicosapentaenoyl phosphatidylcholines by polyethylene glycol-modified lipase in benzene |
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| Authors: | Takayuki Yoshimoto Masahide Nakata Shigehiko Yamaguchi Tadashi Funada Yuji Saito Yuji Inada |
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| Affiliation: | (1) Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguroku, Tokyo 152, Japan |
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| Abstract: | Summary Lipase fromCandida cylindracea was modified with activated polyethylene glycol, which was synthesized from  -carboxymethyl- -methoxypoly-(oxyethylene) and N-hydroxysuccinimide with dicyclohexylcarbodiimide. The modified lipase was soluble in organic solvents such as benzene and catalyzed the reaction of ester-exchange to synthesize eicosapentaenoyl phosphatidylcholines from dipalmitoyl phosphatidylcholine and eicosapentaenoic acid. Either one of palmitic acid at C-1 or C-2 position of the phosphatidylcholine was exchanged with eicosapentaenoic acid. |
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