小鼠负重游泳后血清代谢模式变化分析

目的：研究小鼠负重游泳导致疲劳过程中血清代谢模式改变,为营养抗疲劳研究提供依据。方法：雄性昆明小鼠,随机分为4组,以AIN-93饲料喂养14 d,分别接受0（正常对照）、30、601、20 min等不同时间的尾部负重游泳,游泳后处死小鼠,分离血清进行代谢组分析。采用驰豫编辑脉冲序列（CPMG）和扩散编辑脉冲序列（LED）采集血清样本的数据,经傅立叶变换得到1H NMR谱图,SIMCA-P＋软件进行主成分分析。结果：游泳后小鼠血清代谢组发生显著改变,较正常对照组升高的化合物有β-羟基丁酸、乙酸、乳酸、脂类物质等,降低的化合物有葡萄糖、胆碱、磷酸胆碱、丙氨酸、磷脂酰胆碱等,随游泳时间延长变化趋势相应逐渐增大。结论：小鼠游泳后血清代谢模式产生一些规律性变化,其中脂肪代谢相关物质变化较为显著,针对胆碱类物质在脂肪代谢中的作用开展研究对于揭示疲劳过程本质及其机制具有一定意义。

The metabolic changes of mice serum after loaded swimming

Objective： To investigate the metabolic changes of mice serum after loaded swimming and to provide a basis for the study of anti-fatigue functional food.Methods： The male Kunming mice were randomly divided into four group,fed an AIN-93 diet for 14 days,and forced to swim for 30,60 or 120 min,respectively,with a load on their tails.The mice were executed after swimming immediately and the changes of serum metabolic profiles were analyzed using metabolomic approach.The spectrum was acquired by using Carr Purcell Meiboom Gill（CPMG） or Longitudinal Eddy Current Delay（LED） sequence,and transformed into 1H NMR spectrogram via Fourier transformation.All the data were analyzed by principal component analysis by using the SIMCA-P＋ software.Results： The serum metabolic profiles changed significantly after loaded swimming.Serum β-hydroxybutyric acid,acetate,lactate,lipid were increased and glucose,choline,phosphorylcholine,alanine and phosphatidylcholine decreased.These changes were time dependent.Conclusion： The changes of serum metabolic profiles after loaded swimming were time dependent,especially for lipid metabolite.Further study based on the interaction of choline and lipid metabolism may contribute to understand the mechanism of fatigue.