瘦素促进皮肤创伤大鼠胶原合成的实验研究

目的：研究瘦素对创伤大鼠创面组织胶原合成的影响,探讨瘦素促进创伤愈合的作用机制。方法：雄性Wistar大鼠30只,体质量（180±20）g,按体重随机分为：正常组、创伤对照组和瘦素治疗组,用5%Na2S背部脱毛。瘦素治疗组和创伤对照组大鼠于背部制做缺损创面（2.0 cm×2.5 cm）,并分别用瘦素蛋白溶液0.1 ml（含瘦素2.0μg）和等体积生理盐水涂抹,每天一次,连续7 d。之后处死大鼠,取创面组织及正常组大鼠相应部位脱毛皮肤检测皮肤及创面胶原合成相关指标。结果：瘦素治疗组肉芽组织中羟脯氨酸含量（33.92±3.09）mg/g较创伤对照组（29.55±3.59）mg/g显著升高（P〈0.05）,I、Ⅲ型胶原mRNA的水平0.96±0.09、0.09±0.06分别较创伤对照组0.80±0.03、0.08±0.03显著增加（P〈0.05）,I、Ⅲ型胶原的蛋白表达也较创伤对照组显著增强。结论：瘦素通过促进创伤组织I、Ⅲ型胶原的基因表达和胶原蛋白的合成,从而加快肉芽组织的生长,促进创伤组织的修复和愈合。

Study on leptin enhancing collagen systhesis in wounded rats

Objective： To investigate the effect of leptin on collagen systhesis in wounded rats.Methods： Thirty male Wistar rats,weight（180±20）g,were randomly divided into three groups（n=10） by weight： normal depilation group,wound control group and leptin treatment group and ten rats were included in each group.A full-thickness defect measuring 2×2.5 cm was made in the back of rats in wound control group and leptin treatment group.Each wound in rats of leptin treatment group was applied topically with 0.1 ml leptin solution（2.0 μg leptin）, daily for 7 days and that of wound control group with equivalent saline solution. All rats were killed and then granulation tissues samples and skin were collected to examine the synthesis of collagen.Results： Hydroxyproline content in granulation tissues of in leptin treatment group（33.92±3.09）mg/g were significantly increased than those in control group（29.55±3.59 mg/g,P0.05）.The mRNA expressions of collagen I and Ⅲ were significantly enhanced in leptin treatment group（0.96±0.09、0.09±0.06） than those in control group（0.80±0.03、0.08±0.03）.The levels of type I and Ⅲ collagen were significantly increased in leptin treatment group than those in control group.Conclusion： Leptin applied topically can accelerate wound healing through enhancing gene expression of type I and Ⅲ collagen and synthesis of collagen in wound tissue.