Plant regeneration from immature cotyledon explant cultures of bean (P. coccineus L.)

A method for plant regeneration, via organogenesis and somatic embryogenesis, from P. coccineus is described. Immature cotyledons from plants regenerated and cloned in previous experiments were used. The highest percentage of regeneration (37.5%) was observed from the cotyledons of clone C7 on a modified Murashige & Skoog basal medium to which (2-isopentenyl)adenine (2iP, 10 mg l^-1) and 2-naphthoxyacetic acid (NOA, 0.05 mg l^-1) were added. On average, the same medium gave the highest percentage of regeneration also from the cotyledons of the 7 clones of P. coccineus used in the experiment. Newly regenerated plants were normally fertile. The procedure described may serve for induction of somaclonal variation for in vitro selection of valuable genotypes and for genetic transformation by A. tumefaciens.Abbreviations 6BA 6-benzylaminopurine - CCC chlorocholine chloride - IAA indole-3-acetic acid - 2iP (2-isopentenyl)adenine - NOA 2-naphthoxyacetic acid