Development of human chromosome-specific PCR primers for characterization of somatic cell hybrids |
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Authors: | C Abbott S Povey |
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Affiliation: | Department of Genetics & Biometry, University College London, United Kingdom. |
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Abstract: | The human chromosome complement of somatic cell hybrids must be assessed each time the hybrids are grown in culture. We have developed a panel of human-specific oligonucleotide primers for genes that have been mapped to each of the autosomes and to the X chromosome. These primers enable the human chromosome content of hybrids to be assessed rapidly by PCR. The sequence of the primers is presented together with the appropriate conditions for human DNA-specific amplification for each pair. |
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