Apolipoprotein C-II synthesis as studied in explants of rat liver in culture |
| |
Authors: | M Jauhiainen |
| |
Institution: | 1. Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA;2. Center for Pharmacogenomics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA;3. Departments of Pediatrics and Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA;1. Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan;2. Department of United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan;1. Arizona State University, Tempe, AZ 85287-1504, United States;2. Thomas Jefferson National Accelerator Facility, Newport News, VA 23606, United States;3. University of Connecticut, Storrs, CT 06269, United States |
| |
Abstract: | Adult male Sprague-Dawley rats (200-250 g) were used to study apolipoprotein C-II synthesis and secretion. Liver slices were prepared and incubated in RPMI 1629-medium (tissue amount and incubation time studies) and in Minimum Essential Medium (Eagle) with Earle's salts (hormone experiments). Incubation was performed in scintillation vials in a 95% O2-5% CO2 atmosphere, at 37 degrees C from 1 to 21 hr (2 and 4 hr with hormones). The hormones used and their amounts per millilitre medium were: oestradiol-17 beta 0.1 microgram, progesterone 3.0 micrograms and dexamethasone 1.5 micrograms. Apolipoprotein C-II was determined by specific double immunoprecipitation technique and TCA-insoluble protein fraction represented total protein. Optimal tissue amount was 100 mg/vial and the results show that liver slices quickly secrete the newly synthesized apo C-II (also total protein) into the surrounding medium. There were only minor differences between apo C-II values with the hormones used. The portion of apo C-II synthesis from total protein synthesis was 0.47-1.50%. After 4 hr incubation the 3H]leucine incorporation was almost equal for controls and hormone treated slices. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|